any changes done on the tune can be done without re-cycling the power. So the answer is No.
'95 Probe gt, Twincharged T66 turbo/M62 clutched blower on 15psi -No longer exists
'00 Hyundai Accent 2.0L-T on Ms1 ver 2.2 running and needing tuned
'14 Factory Five 818s on Cobb - mid life chrisis
'93 Mercury Tracer 2.5L v6 mazda swap, under construction - replaced the Probe